Figure 6.

Phosphorylation activity of GST:PID. A, Autophosphorylation of GST:PID (first lane), and phosphorylation of MBP (second lane). No phosphorylation of PBP1 or TCH3 was observed (third and fourth lanes, respectively). B, Parallel nonlabeled protein samples were run on a gel and were checked for the presence of the two His-tagged-interacting proteins by western blotting using anti-His antibodies. C and D, A second protein gel stained with Coomassie Blue detects MBP (C) and shows equal loading of the GST:PID fusion protein (D).