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SDS-PAGE analysis of the 3′GT protein purified from gentian petals. The active fractions after each purification step were analyzed through a 10% to 20% (m/v) polyacrylamide gel and detected by silver-staining. Lane M, Mr marker; lane 1, fraction of 40% to 70% saturation with ammonium sulfate; lane 2, Sephadex G-25 elute; lane 3, Q-Sepharose elute; lane 4, Blue A elute; lane 5, Superose 12 elute. Mr for each band of the marker is indicated to the left of the panel.
