Figure 3.

HPLC analysis of the reaction products of the purified gentian 3′GT. A, HPLC profiles of the substrate DEL 3G-5G (left) and the reaction product by the purified gentian 3′GT using DEL 3G-5G and UDP-Glc as substrates (right). A₅₂₀ was monitored. The retention times corresponding to DEL 3G-5G and the major product were approximately 14.6 and 11.4 min, respectively. B, UV-visible spectra of the DEL 3G-5G (solid line) and the major product detected at 11.4 min in HPLC analysis (dashed line). The absorbance maximum for DEL 3G-5G and the major product were 524 and 515 nm, respectively (as indicated).