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1H- and 13C-NMR assignment and structure of the reaction produced by 3′GT. A, Assignment of the NMR spectra of the reaction product by the recombinant 3′GT protein using DEL 3G-5G and UDP-Glc as substrates. B, Structure of the reaction product confirmed by ROESY and heteronuclear multiple bond correlation (HMBC) analyses. Rotating frame overhauser enhancement (ROE) detected on ROESY are indicated by arrow curves and HMBC cross peaks are indicated by thick lines.
