Table I.
Subcellular distribution of recombinant LePSD activity in yeast
PSD activity was measured using with NBD-Ptd-[1′-14C]Ser as substrate. Data are means of three replicates ± se.
Construct
|
PSD Activity
|
||
---|---|---|---|
Mitochondria | Microsomes | Cytosol | |
nmol min-1 mg-1 protein | |||
Yeast PSD1 | 0.658 ± 0.063 | 0.02 ± 0.008 | <0.001 |
Vector control | <0.001 | <0.001 | <0.001 |
Full-length LePSD1 | 0.127 ± 0.048 | 0.003 ± 0.002 | <0.001 |
Truncated LePSD1 | 0.550 ± 0.218 | 0.136 ± 0.033 | 0.004 ± 0.002 |
Chimeric LePSD1 | 0.872 ± 0.222 | 0.150 ± 0.067 | 0.001 |