Table II.
Quantification of AtPSD1 mRNA in organs of wild-type Arabidopsis and a knockup mutant
Levels of mRNA were determined by real-time quantitative RT-PCR, using an amplicon spanning exons 10 and 11 of the AtPsd1 gene. Roots were either from hydroponically grown plants, or from in vitro cultures in Murashige and Skoog medium. Three independent RNA extracts were made of each organ, and triplicate mRNA determinations were made on each extract. Data are means of all nine determinations ± se. An internal AtPSD1 RNA standard (6.2 pg) was used to estimate recovery from RT-PCR of each sample; recovery values were 90% ± 4%. Data are corrected for recovery.
| Genotype | Organ | AtPSD1 mRNA |
|---|---|---|
| fg 250 ng-1 total RNA | ||
| Wild type | Young leaves | 121 ± 13 |
| Mature leaves | 94 ± 16 | |
| Stems + flowers | 106 ± 10 | |
| Roots (hydroponic) | 228 ± 12 | |
| Roots (Murashige and Skoog medium) | 143 ± 13 | |
| Homozygous mutant | Young leaves | 1605 ± 378 |
| Mature leaves | 740 ± 76 | |
| Roots (Murashige and Skoog medium) | 787 ± 29 |