Figure 7.

Expression of Trx h3 and h4 isoforms in seeds and seedlings by quantitative PCR. cDNAs produced by RT of total RNAs from cotyledons and embryo axes from seeds imbibed for 0 to 46 h (UG, ungerminated seeds; G, germinated seeds) and from green leaves (GL) and roots (R) from 7-d-old seedlings were used as templates in quantitative PCR realized in the presence of Sybr Green. Copy number of each isoform was determined using a standard curve established with the recombinant plasmid containing the corresponding full-length sequence.