Figure 10.

SMART cDNA Gel Blot Hybridization.

SMART cDNA from 3-week-old seedlings of wild-type Micro-Tom (lanes 1) or ANT1 transgenic Micro-Tom, event BK (lanes 2), was separated, transferred, and probed with a radiolabeled DNA fragment corresponding to actin and each labeled gene. In all cases, the transcripts that were upregulated in the ANT1 transgenic tomato were undetectable in the untransformed control. Note the putative alternate polyadenylated forms of tomato DFR transcript. The numbers (in kb) indicate the estimated molecular masses of the hybridizing bands.