Figure 4.

Alignment of Predicted Protein Sequences of ANT1 and Related MYB Factors.

(A) Amino acid alignment of the R2R3 MYB domain of functionally characterized plant proteins involved in the regulation of flavonoid biosynthesis. The tomato MYB factor identified in this study (LeANT1) is compared with petunia AN2, Arabidopsis PAP1, Arabidopsis PAP2, Arabidopsis TT2, maize Pl, and maize C1. Residues that are identical to residues in the tomato ANT1 sequence are shaded. The positions of the conserved W/I residues implicated in DNA binding are indicated below the alignment with asterisks.

(B) Amino acid sequence alignment of the full-length tomato ANT1 and petunia AN2 proteins showing identical (asterisks) and similar (dashes) residues (CLUSTAL W, version 1.4: open gap penalty of 10.0; extended gap penalty of 0.1; similarity matrix of blosum62).