Table 3.
Genes That Are Upregulated in ANT1 Transgenic Tomato
| SSH Clone Name |
SSH Insert Size (bp) |
EST Contig Size (bp) |
ESTs in the Contig (GenBank Accession Number) |
BLASTN Score (P Value) |
Putative Identity |
|---|---|---|---|---|---|
| MT13 | 1004 | 498 | BE462282, BE462229, AW626121 | 1232 (4.9e-51) | MYB factor, similar to petunia AN2 (=ANT1) |
| MT16 | 480 | 1410 | BM411645 | 2382 (2.0e-103) | CHS |
| MT2 | 546 | 1667 |
BE461567, BE460511, BE459234, BE436963, BE436886, BE436794, BE436417, BE436385, BE436300, BE436296, BE436116, BE435816, BE435361, BE435208, BE435015, BE434398, BE434141, BE434084, BE433520, BE433099, BE432679, BE432054, BE431801, AW933199, AW932099, AW931633, AW931609, AW930557, AW651280, AW651250, AW650795, AW648641, AW442216, AW442098, AW216889, AW220654, AW220655, AW220656, AW220874, AW221860, AW222352 |
941 (2.0e-38) | 5-O-Glucosyltransferase |
| MT11 | 281 | 673 | BI209061 AI775693 BG628982 BG631865 BG630259 | 1400 (9.3e-59) | 3-O-Glucosyltransferase |
| MTP4 | 400 | 521 | AI778224 | 730 (2.2e-28) | Type-I GST, similar to petunia AN9 |
| MTP96 | 970 | 831 | BQ505699 | 1753 (1.1e-72) | Similar to CHI |
| MTP2 | 301 | 362 | AI896332 | 1463 (2.5e-61) | HD-GL2, similar to ANL2 |
| MTP77 | 390 | 620 | BE354224 | 816 (2.4e-32) | Permease, similar to TT12 (and family) |
SSH fragments were cloned and sequenced. The SSH fragment sequence was compared with a database containing tomato ESTs assembled into the least number of contigs (BLASTN). The EST contig with the greatest match to the SSH fragment was assigned a putative identity based on a BLASTX search against the nonredundant protein database in GenBank (Altschul et al., 1997).