Figure 7.

Immunological Characterization of ZmHda1 Forms during Maize Embryo Germination.

(A) Embryos were harvested into liquid nitrogen at various times during embryo germination (hours after the start of imbibition). Proteins were extracted, and equal amounts of protein were subjected to SDS-PAGE. The gel was blotted onto a nitrocellulose membrane, and proteins were immuno- detected with anti-ZmHda1 antibodies (peptide antibodies). Arrows mark the positions of the high molecular weight ZmHda1 form and the 48-kD form.

(B) The same blot shown in (A) was immunoblotted with antibodies against the C-terminal part of ZmHda1. The arrow marks the position of the high molecular weight ZmHda1 form, and the asterisk marks the position of the cleaved C-terminal peptide.