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Analyses of HCV NS5B proteins. (A) Sequence comparison of the NS5B proteins used in this study. The C-terminal domain deleted from NS5B-540H is highlighted. (B) Analysis of purified HCV NS5B proteins. A 200-ng portion of purified NS5B protein was subject to separation by SDS-PAGE followed by Coomassie blue staining. Lanes: 1, NS5B-570H; 2, NS5B-540H. (C) CD spectrometric analysis of NS5B-570H (solid line) and NS5B-540H (dotted line).
