FIG. 10.

Identification of a 200-bp region within KSHV Ori-Lyt that associates with the C/EBPα protein. (A) Schematic diagram showing the map location of all DNA probes used from the duplicated KSHV Ori-Lyt domain as well as the subregion containing multiple CTF motifs used for EMSA experiments and the identified C/EBP binding sites relative to the two AT-rich palindromes (bottom line). (B) Results of EMSA experiments testing the abilities of seven DNA probe fragments from across the Ori-Lyt domain to bind RAP or C/EBPα proteins. (C) Results of EMSA analysis to detect binding of C/EBPα or RAP to wild-type or mutated ³²P-labeled oligonucleotide probes encompassing the four putative paired CTF motifs.