FIG. 6.
Ad-RAP also induces C/EBPα and p21 protein expression in human DMVEC. (A and B) G1 arrest as judged by double-label IFA showing the absence of BrdU incorporation (rhodamine, red) in RAP-positive cells (FITC, green) but not in RAP-negative cells of a DMVEC culture after infection with Ad-RAP(1-237) for 40 h at an MOI of 1.0. (C) DAPI nuclear staining of all DMVEC cells in the same field as panels A and B. (D and E) Double-label IFA showing the expression of RAP (green) and induced p21 (red) in the same subpopulation of cells infected with Ad-RAP. (F) Merge of panels D and E, showing that p21 is induced only in RAP-positive cells. (G and H) Double-label IFA illustrating the expression of RAP (green) and upregulation of C/EBPα (red) in the same subpopulation of cells after Ad-RAP infection. (I) Merge of panels G and H illustrating coexpression of C/EBPα only in RAP-positive cells.