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Monitoring of protein E release (A) and proportion of protein E-producing cells (B) during the derivation of CHO-ME cells. Protein E was quantified 72 h postseeding of cells by SDS-ELISA. Protein E-producing cells were identified by immunofluorescence staining. 1, cells derived from a single-cell colony after transfection of CHO-DG44 cells with the expression plasmids; 2, cells after the first subcloning step; 3, CHO-ME cells as derived after a second subcloning step.
