TABLE 1.

Primers used for the construction of cDNA clones

Primer	Orientation	Sequence, 5′-3′a	Positionb
P-ΔCME1	Sense	CCGACGCGTGGAAGACGATCGGATCCTACAATGTCCATGAGCTTTCT	2392-2414
P-ΔCME2	Antisense	GTGACCGAGCTTCTCCACAT	2659-2678
P-ΔME1	Sense	ACGTGTTGAGAAAAAGACAG	91-110
P-ΔME2	Antisense	ATCGGATCCGTCGCTGACCTCCTTTTC	411-431

^aRecognition sites for restriction enzymes are underlined (MluI, ACGCGT; BamHI, GGATCC). Additional nucleotides introduced for plasmid constructions are in italics.

^bPosition numbers of matching nucleotides correspond to the wild-type virus genome sequence (GenBank accession no. U27495).