FIG. 2.

Induction of MxA is delayed in BRSV ΔNS1-infected cells. MDBK cells were mock infected or infected at an MOI of 0.2 with BRSV wt, BRSV ΔΝS1, or BRSV ΔΝS2. When indicated, 2,000 U of IFN-α A/D per ml was added immediately after infection. Cells were harvested 16, 24, and 40 h postinfection, and equal amounts of cell extract were subjected to SDS-PAGE followed by detection of MxA, BRSV N and P protein, and PCNA.