FIG. 3.

BRSV wt does not interfere with IFN signaling and induction of ISGs by IFN. MDBK cells were mock infected or infected at an MOI of 0.2 with BRSV wt. When indicated, 2,000 U of IFN-α A/D per ml was added immediately after infection. Cells were harvested 24 or 48 h postinfection, and equal amounts of cell extract were subjected to SDS-PAGE followed by detection of MxA, BRSV N protein, and PCNA.