FIG. 1.

UV cross-linking of ³²P-labeled t2 RNA (3′ 195 nt of TMV-L RNA) to purified TMV-L RdRp. Purified TMV-L RdRp was incubated with ³²P-labeled t2 RNA probe and UV irradiated. The product was analyzed by SDS-PAGE (21) and autoradiography. Lane 1, probe (no UV); lane 2, probe (UV irradiated); lane 3, probe plus TMV-L RdRp (UV irradiated). 183K and 126K, the positions of the TMV-L 183- and 126-kDa proteins, respectively; C, the position of the cross-linked product; PROBE, the position of the labeled t2 RNA probe.