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. 2003 Aug;77(16):8669–8675. doi: 10.1128/JVI.77.16.8669-8675.2003

FIG. 7.

FIG. 7.

Effect of mutations of aromatic amino acids to alanine in P1 on ability to cross-link to t2 RNA. (A) SDS-PAGE of soluble, uncross-linked proteins. Lane M, marker proteins (120, 100, 90, 80, 70, 60, and 50 kDa); lane W, wild-type P1; lane 1, Y333A; lane 2, W365A; lane 3, F366A; lane 4, F378A; lane 5, F398A; lane 6, F409A; lane 7, Y416A; lane 8, F422A. The gel was stained with Coomassie brilliant blue. The sizes (in kilodaltons) of selected protein marker bands are shown on the side of the gel. (B) UV cross-linking of 32P-labeled t2 RNA with P1 mutants. After UV cross-linking, the products were analyzed by SDS-PAGE and phosphorimaging. Lane W, wild-type P1; lane 1, Y333A; lane 2, W365A; lane 3, F366A; lane 4, F378A; lane 5, F398A; lane 6, F409A; lane 7, Y416A; lane 8, F422A.