FIG. 6.

Expression and N-linked glycosylation of TfRs from feline (NLFK and CRFK) and canine (A72, Cf2Th, and Walter Reed) cells or of wild-type or mutant TfRs expressed from plasmids by transfection of TRVb cells. Proteins were collected by lysis of the cells and then were either not treated or incubated with PNGase before electrophoresis on 10% acrylamide gels and detection of the TfR by Western blotting. (A) The feline or canine TfR recovered from cell lines of feline or canine origin or after expression from cDNA clones in hamster TRVb cells. Samples were either not treated or were treated with PNGase before electrophoresis. (B) The wild-type feline and canine TfRs and mutants containing an insertion (ins) or deletion (del) of Asn at position 205 and with an Asn or Lys at position 383 to alter those positions to the sequence of the alternative host receptor. All plasmids were expressed in TRVb cells.