FIG. 5.

Map of PML isoform IV (633 amino acid residues [aa]), showing the RING finger (R), the two B boxes (B1 and B2), the coiled-coil region (CC), the nuclear localization signal (nls), and the two major characterized sites of SUMO-1 modification, lysine 160 and lysine 490. At the top are shown the positions of restriction sites in the cDNA that were used for the generation of the truncated derivatives depicted below. The addition of nls to the name of a truncated plasmid indicates that the simian virus 40 large-T-antigen nuclear localization signal had been added to the C-terminal end of the open reading frame.