FIG.6.

Effect of Tip interactions with p80 and Lck on Tip-mediated alteration of lipid raft and CD3 localization. Jurkat T cells were electroporated with an expression vector expressing wild-type (wt) Tip, Tip Y₁₁₄S, Tip Δ2, or Tip mSH3B. At 48 h postelectroporation, cells were fixed and reacted with rabbit polyclonal anti-Tip antibody (red), mouse monoclonal anti-CD3 antibody (blue), and CT-B (green). Yellow in the merged panel indicates colocalization of the red and green labels. Immunofluorescence was examined with a Leica confocal immunofluorescence microscope, and representative optical sections are presented.