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. 2003 Aug;77(16):8985–8999. doi: 10.1128/JVI.77.16.8985-8999.2003

FIG. 2.

FIG. 2.

Expression of nectin-1 and nectin-1-GFP proteins in cell lines derived from B78H1 cells. Nectin-1 in B78H1 and C10 cells was detected using MAb CK41 coupled with Oregon Green (A and C), whereas nectin-1-GFP in CG23, CXG10, and NGC12 cells was observed directly by green fluorescence (E, G, and I). In these permeabilized cells, afadin was detected with anti-AF6 MAb followed by alexa594-coupled secondary antibody (B, D, F, H, and J). Representative pictures of various patterns are shown. These patterns are not cell-type specific and represent cell contact surfaces viewed from different angles (enlarged in panels E and F). Images A to F were collected on a regular fluorescence microscope (Nikon Eclipse E600), and images G to J were collected on a confocal microscope (Nikon TE-300 inverted microscope coupled to a Bio-Rad Radiance 2000-MP confocal imaging system).