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In vitro replication of VSV-IFNβ in Stat1+/+ or Stat1−/− cells. Immortalized embryonic fibroblasts derived from Stat1+/+ (A and B) or Stat1−/− (C and D) C57BL/6 mice were treated with (solid bars) or without (open bars) murine IFN-α/β (1,000 U/ml) for 24 h and were mock infected or infected with VSV-IFNβ or VSV-GFP at an MOI of 0.01 PFU per cell for 24 h. Cell viability was assessed by trypan blue exclusion assay (A and C) or visually by microscopy (magnification, ×20) (B and D).
