FIG. 2.

Intracellular distribution of yeast tRNA^Phe mutants following transfection. Northern blots of 5 μg of cytoplasmic RNA (top) or total RNA (bottom) collected from HeLa H1 cells after transfection with pU6^PheWt, pU6^PheD⁻, or pU6^PheUUA are shown. Lanes 1, RNA from HeLa cells transfected with pU6^PheWt; lanes 2, RNA from HeLa cells transfected with pU6^PheD⁻; lanes 3, RNA from HeLa cells transfected with pU6^PheUUA. The Northern blots were probed for yeast tRNA^Phe and mammalian tRNA^Lys as depicted; the U6 snRNA probe served as a control to ensure that no nuclear RNA was present in the samples.