FIG. 5.
Complementation of MuLV with yeast tRNAPhe mutants. (A) Numbers of CFU (drug-resistant colonies) derived from infection of pMuLV-Phe complemented by yeast tRNAPheWt or yeast tRNAPheUUA expressed from 1.5 μg of cotransfected tRNAPhe mutant plasmids (tRNAPhe DNA) or in vitro-transcribed tRNAPhe (tRNAPhe RNA). A plasmid expressing tRNAPro from the U6 promoter was used for complementing MuLV-Phe as a negative control. The presented data were collected from three independent complementation experiments, and standard deviations are provided. (B) Complementation with tRNAPheD−. The plasmid expressing tRNAPheD− or in vitro-transcribed tRNAPheD− was cotransfected with pMuLV-Phe. The number of drug-resistant colonies was determined after infection. Under these conditions, the number of CFU for tRNAPheD− (DNA) was 1 or 2 colonies, which was the background level seen with a vector clone.