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Coexpression of F13L with a dominant-negative Eps15 mutant. HeLa cells were transfected or cotransfected with plasmids expressing F13L-HA, D3Δ2, or EH21. After 24 h, the cells were incubated with TR-Tfn for 20 min and then either fixed and examined by confocal microscopy or first permeabilized and stained with an anti-HA MAb followed by rhodamine red-conjugated anti-mouse IgG. Bars, 10 μm.
