FIG. 6.
Coimmunoprecipitation of F13L with α-adaptin. BS-C-1 cells infected with vF13LHAC were harvested and lysed. The lysate was incubated with an antibody to α-adaptin, clathrin, or γ-adaptin, and immune complexes were captured on protein A beads, resolved by SDS-PAGE, and transferred to a nitrocellulose membrane. The membrane was probed with an anti-HA polyclonal antibody to detect the F13L protein. A sample of the cell lysate was also analyzed directly. The masses of marker proteins (in kilodaltons) are given on the left.