FIG. 8.

Effect of nocodazole treatment on the colocalization of F13L-GFP with LAMP2. HeLa cells were transfected with pF13L-GFP or infected with vF13L-GFP. At 24 h after transfection or 18 h after infection, cells were either left untreated or treated with nocodazole (Noc) for 30 min. The cells were then fixed, permeabilized, and stained with an anti-LAMP2 MAb followed by rhodamine red-conjugated anti-mouse IgG. Arrows point to the overlap of F13L-GFP with LAMP2. Green, GFP; red, rhodamine red; yellow, overlap of green and red. Bars, 10 μm.