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. 2006 Nov 30;103(50):18928–18933. doi: 10.1073/pnas.0606379103

Fig. 3.

Fig. 3.

NSAF analysis of RNA pol II components copurifying with human Mediator. (A) NSAF values for RNA pol II components are plotted for each of the baits used to purify Mediator. From left to right above each bait is the average NSAF value (with error bars) of Rpb1 to Rpb12 in numerical order. RNA pol II components in F-Med26 and F-Med10 purifications that were statistically different (P < 0.05) from all other preparations are represented by a vertical ∗∗, and RNA pol II components in F-Med26 and F-Med10 preparations that were only significantly different from F-Med28 and F-Med29 preparations are represented by ∗. Rpb7 in F-Med10 was significantly different from F-Med26 and F-Med29 but not F-Med28, and this is represented by + (see SI). (B) Quantitative Western blotting of the RNA pol II components Rpb1 and Rpb9 from each of the affinity-purified Mediator preparations. The Med17 Western blot analysis is the same as shown in Fig. 1 and is provided as a frame of reference. (C) Basal transcription performed on a naked DNA template with and without added RNA pol II. The amount of added eluate was normalized to Med17 subunit as determined from Western blot analysis. Transcription from the samples without added RNA pol II could only come from RNA pol II present in the Mediator preparations.