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. 1995 Apr;61(4):1641–1642. doi: 10.1128/aem.61.4.1641-1642.1995

Detection of Lyme disease spirochetes in the skin of naturally infected wild sika deer (Cervus nippon yesoensis) by PCR.

K Kimura 1, E Isogai 1, H Isogai 1, Y Kamewaka 1, T Nishikawa 1, N Ishii 1, N Fujii 1
PMCID: PMC167423  PMID: 7747979

Abstract

We demonstrated the presence of Borrelia burgdorferi sensu lato DNA in the skin tissues of naturally infected wild sika deer, using PCR. The risk of transmission of B. burgdorferi sensu lato is recognized in sika deer.

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Selected References

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  1. Anderson J. F., Magnarelli L. A. Avian and mammalian hosts for spirochete-infected ticks and insects in a Lyme disease focus in Connecticut. Yale J Biol Med. 1984 Jul-Aug;57(4):627–641. [PMC free article] [PubMed] [Google Scholar]
  2. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J. C., Assous M., Grimont P. A. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol. 1992 Jul;42(3):378–383. doi: 10.1099/00207713-42-3-378. [DOI] [PubMed] [Google Scholar]
  3. Barbour A. G. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed] [Google Scholar]
  4. Bosler E. M., Ormiston B. G., Coleman J. L., Hanrahan J. P., Benach J. L. Prevalence of the Lyme disease spirochete in populations of white-tailed deer and white-footed mice. Yale J Biol Med. 1984 Jul-Aug;57(4):651–659. [PMC free article] [PubMed] [Google Scholar]
  5. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. Lyme disease-a tick-borne spirochetosis? Science. 1982 Jun 18;216(4552):1317–1319. doi: 10.1126/science.7043737. [DOI] [PubMed] [Google Scholar]
  6. Isogai E., Isogai H., Masuzawa T., Yanagihara Y., Sato N., Hayashi S., Maki T., Mori M. Serological survey for Lyme disease in sika deer (Cervus nippon yesoensis) by enzyme-linked immunosorbent assay (ELISA). Microbiol Immunol. 1991;35(9):695–703. doi: 10.1111/j.1348-0421.1991.tb01603.x. [DOI] [PubMed] [Google Scholar]
  7. Isogai E., Tanaka S., Braga I. S., 3rd, Itakura C., Isogai H., Kimura K., Fujii N. Experimental Borrelia garinii infection of Japanese quail. Infect Immun. 1994 Aug;62(8):3580–3582. doi: 10.1128/iai.62.8.3580-3582.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jaenson T. G., Tälleklint L. Incompetence of roe deer as reservoirs of the Lyme borreliosis spirochete. J Med Entomol. 1992 Sep;29(5):813–817. doi: 10.1093/jmedent/29.5.813. [DOI] [PubMed] [Google Scholar]
  9. Lane R. S., Piesman J., Burgdorfer W. Lyme borreliosis: relation of its causative agent to its vectors and hosts in North America and Europe. Annu Rev Entomol. 1991;36:587–609. doi: 10.1146/annurev.en.36.010191.003103. [DOI] [PubMed] [Google Scholar]
  10. Miyamoto K., Nakao M., Sato N., Mori M. Isolation of Lyme disease spirochetes from an ixodid tick in Hokkaido, Japan. Acta Trop. 1991 Apr;49(1):65–68. doi: 10.1016/0001-706x(91)90031-e. [DOI] [PubMed] [Google Scholar]
  11. Miyamoto K., Nakao M., Uchikawa K., Fujita H. Prevalence of Lyme borreliosis spirochetes in ixodid ticks of Japan, with special reference to a new potential vector, Ixodes ovatus (Acari: Ixodidae). J Med Entomol. 1992 Mar;29(2):216–220. doi: 10.1093/jmedent/29.2.216. [DOI] [PubMed] [Google Scholar]
  12. Oliver J. H., Jr, Stallknecht D., Chandler F. W., James A. M., McGuire B. S., Howerth E. Detection of Borrelia burgdorferi in laboratory-reared Ixodes dammini (Acari: Ixodidae) fed on experimentally inoculated white-tailed deer. J Med Entomol. 1992 Nov;29(6):980–984. doi: 10.1093/jmedent/29.6.980. [DOI] [PubMed] [Google Scholar]
  13. Picken R. N. Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever. J Clin Microbiol. 1992 Jan;30(1):99–114. doi: 10.1128/jcm.30.1.99-114.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Telford S. R., 3rd, Mather T. N., Moore S. I., Wilson M. L., Spielman A. Incompetence of deer as reservoirs of the Lyme disease spirochete. Am J Trop Med Hyg. 1988 Jul;39(1):105–109. doi: 10.4269/ajtmh.1988.39.105. [DOI] [PubMed] [Google Scholar]
  15. Wilson M. L., Telford S. R., 3rd, Piesman J., Spielman A. Reduced abundance of immature Ixodes dammini (Acari: Ixodidae) following elimination of deer. J Med Entomol. 1988 Jul;25(4):224–228. doi: 10.1093/jmedent/25.4.224. [DOI] [PubMed] [Google Scholar]

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