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. 2006 Nov 27;5:35. doi: 10.1186/1475-2859-5-35

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Copyright © 2006 Castaldo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transcriptional analysis of class I heat shock operons. Primer extension analysis of: (A) groESL, (B) dnaK, and (C) lp_rRNA01 transcripts. Primer extension products were obtained by using oligonucleotides groESL2, hrc2, and sed2, respectively. Total RNA was extracted from: LM3 cells grown at 30°C (lane 1), or after a 15 min heat shock at 50°C (lane 2); LM3-2 cells grown at 30°C (lane 3), or after a 15 min heat shock at 50°C (lane 4). As a reference, sequencing reactions were performed with the same primers.