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Effect of adding 5 μm nitrite to the incubation medium on the cytoplasmic and the vacuolar pH values of root segments grown and incubated without nitrate. The switch to anoxia occurred at time zero, the dotted lines connect the normoxic and anoxic pH values, and each pH value is the mean of at least three replicate measurements ±se. □, No nitrite; ⋄, 5 μm nitrite.
