. 2006 Dec;142(4):1603–1620. doi: 10.1104/pp.106.086231

Table III.

Protein spots responding to PAMPs

A change in spot density is classified as PAMP associated if the DC3000 hrpA inoculation is significantly different from the MgCl₂-treated control regardless of the other two treatments. A protein spot is classified as PAMP associated and modulated by T3E if there were significant differences between DC3000 and DC3000 hrpA mutant inoculation and the latter was also significantly different from MgCl₂ treatment.

Gene	Protein^a	Fraction Time^b	Spot Density Treatment^c
Gene	Protein^a	Fraction Time^b				Mg	hrpA	DC3000	avrRpm1
Proteins decreasing in spot density in response to PAMPs		hpi
At4g09650	H⁺-ATPase-related protein	Sol 1.5	6,357^a	4,479^b	5,695^a ^b	4,442^b
At3g55800	Sedoheptulose-bisphosphatase	Sol 4	9,844^a	3,464^b	5,563^b	6,739^b
At1g13440	G3PDH	Sol 4	8,188^a	6,050^b	4,229^b,c	3,044^c
At3g14210	Lipase	Sol 4	15,398^a	9,977^b	10,067^a ^b	11,383^a ^b
At3g14210	Lipase	Sol 6	12,974^a	5,343 ^b	8,765^b
At1g53240	MDH (NAD)	Sol 6	1,587^a	1134^b	1,167^a ^b
At3g26520	Aquaporin TIP1.2	Mit 3	6,179^a	1,062^b	1,197^b	2,083^b
At5g15090	Outer mitochondrial membrane protein porin 2	Mit 3	11,377^a	5,788^b	6,866^b	6,606^b
Proteins decreasing in response to PAMPs modulated by T3E
At3g11630	2-cys-PrxA	Sol 1.5	1,760^a	280^b	1,071^a ^c	654^c
At3g01500	CA, chloroplast	Sol 1.5	1,504^a	750^b	1,310^a	1,676^a ^b
At3g62030	Peptidylprolyl isomerase (ROC4 cyclophilin )	Sol 6	1,764^a	1,017^b	1,754^a
At1g32060	PRK	Cpl 6	1,910^a	509^b	1,706^a
Proteins increasing in response to PAMPs
At1g20020	FNR	Sol 1.5	515^a	910^b	879^a ^b	512^a
At1g02930	GST	Sol 1.5	1,363^a	2,373^b	2,071^a ^b	1,462^a ^b
At5g06290	2-cys-PrxB	Sol 3	975^a	1,212^a ^b	1,330^b	1,318^b
At4g23670	MLP (related, MLP)	Sol 3	1,372^a	2,151^b	2,535^b	2,094^b
At1g02930	GST (GSTF6)	Sol 3	1,823^a	2,736^b	2,976^c	2,663^b
At1g02920	GST (GSTF7)	Sol 3	1,223^a	2,578^b	2,521^a ^b	2,258^a ^b
At1g02920	GST (GSTF7)	Sol 4	910^a	2,861^b	3,430^b	3,074^b
At3g52960	PrxIIE	Sol 4	1,815^a	2,449^b	2,595^b	2,677^b
At1g02930	GST (GSTF6)	Sol 4	2,210^a	3,640^b	3,822^b	3,491^a ^b
At4g02520	GST (GSTF2)	Sol 4	1,080^a	2,433^b	1,550^a ^b	2,081^b
At1g02920	GST (GSTF7)	Sol 6	2,244^a	4,444^b	4,161^b
At1g02930	GST (GSTF6)	Sol 6	2,757	4,985^b	4,633^b
At4g02520	GST (GSTF2)	Sol 6	1,704^a	2,659^b	2,100^a ^b
At1g09340	UDP-Glc 4-epimerase-like	Cpl 3	584^a	1,478^b	1,335^a ^b	522^a ^b
At3g15020	MDH	Mit 3	1,472^a	2,492^b	2,530^a ^b	2,643^a ^b
At4g08900	Putative arginase	Mit 3	0^a	411^b	3,26^b	759^b
At5g14740	CA2	Mit 3	4,748^a	7,784^b	6,431^a ^b	3,852^a ^b
At4g36690	Splicing factor-like protein	Mit 3	397^a	1,400^b	1,288^b	1,299^a ^b
At5g50850	PDH E1β-subunit	Mit 3	77^a	1,322^b	1,544^b	1,325^a ^b
At4g03280	Rieske Fe-S protein	Mit 3	0^a	1,287^b	807^b	1,164^b
At2g39730	Rubisco activase	Mit 3	0^a	1,795^b	1,867^b	1,566^b
Proteins increasing in response to PAMPs modulated by T3E
At3g10920^d	MSD1	Sol 1.5	375^a	662^b	748^b	307^a
At2g47730^d	GST (GSTF8)	Sol 1.5	334^a	639^b	725^b	344^a
At1g53240	MDH [NAD]	Sol 3	599^a	1,330 ^b	755^a	801^a ^b
At5g08280	Hydroxymethylbilane synthase	Sol 4	0^a	1,248^b	561^a	379^a
At4g01050	Hyp-rich glycoprotein	Cpl 1.5	853^a	1,499^b	1,045^a	1,501^b

Proteins are sorted by fraction, time after inoculation, and modulation by T3Es.

Subcellular fraction from which the protein was recovered. sol, Soluble; cpl, chloroplast; mit, mitochondria enriched. Time (hpi) at which the significant change was determined.

Averaged spot density over three replicates. Significant differences (labeled a, b, and c) were determined by t test (P ≤ 0.05). Mg, 10 mM MgCl₂ treatment; hrpA, DC3000 hrpA treated; D3000, DC3000 treated; avrRpm1, DC3000 (avrRpm1) treated.

Proteins that appear to be modulated by AvrRpm1 but not DC3000 T3E. They are included in this table to facilitate discussion in the text.