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. 2006 Dec;142(4):1603–1620. doi: 10.1104/pp.106.086231

Table IV.

Proteins responding to T3Es

A change in spot density is classified as T3E if the DC3000 inoculation was significantly different from the DC3000 hrpA inoculation regardless of the other two treatments. A protein spot is classified as T3E and modulated by AvrRpm1 if the DC3000 inoculation was significantly different from the DC3000 (avrRpm1) treatment and DC3000 was significantly different from DC3000 hrpA.

Gene Proteina Fraction Timeb Spot Densityc
Mg hrpA DC3000 avrRpm1
Proteins increasing in spot density hpi
    At3g01480 Putative thylakoid lumen rotamase (cyclophilin) Sol 1.5 1,360ab 872a 2,167b 1,576ab
    At5g19510 Elongation factor-1B α-subunit Sol 1.5 1,454ab 1,238a 1,613b 1,110ab
    At1g20980 SPB-like 2 Sol 3 448a 449a 630b 528ab
    At1g32060 PRK Sol 3 3,517ab 2,058a 3,528b 2,808ab
    At1g20020 FNR Sol 6 728ab 397a 657b
    At4g32260 H+-ATPase-like protein Cpl 6 1,723ab 425a 1,566b
Proteins decreasing in spot density
    At2g39730 Rubisco activase Sol 4 3,308ab 3,728a 2,118b 2,547b
    At2g37660 3-β-Hydroxysteroid dehydrogenase/isomerase Sol 6 1,096ab 1,784a 1,162b
    At3g04790 Rib-5-P isomerase Sol 6 4,194ab 5,846a 3,347b
Protein increasing in spot density modified by RPM1/AvrRpm1
    At3g16640 TCTP homolog Sol 1.5 1,176ab 704a 1,178b 637a
    At5g10450 14-3-3-like protein AFT1 Sol 1.5 459a 413a 900b 541a
    At5g09650 Inorganic pyrophosphatase-like protein Sol 3 697ab 498a 893b 642a
Protein decreasing in spot density modified by RPM1/AvrRpm1
    At5g66570 OEC33 K protein Cpl 1.5 12,512ab 10,150a 6,614b 11,780a
a

Proteins are sorted by fraction, time after inoculation, and modulation by T3Es.

b

Subcellular fraction from which the protein was recovered. sol, Soluble; cpl, chloroplast; mit, mitochondria enriched. Time (hpi) at which the significant change was determined.

c

Averaged spot density over three replicates. Significant differences (labeled a, b) were determined by t test (P ≤ 0.05). Mg, 10 mM MgCl2 treatment; hrpA, DC3000 hrpA treated; D3000, DC3000 treated; avrRpm1, DC3000 (avrRpm1) treated.