. 2006 Dec;142(4):1621–1641. doi: 10.1104/pp.106.088781

Table I.

Protein spots identified with MALDI-TOF and/or LC-MS/MS

Mass spectrometric identification of protein spots with differential accumulation after OS elicitation using comparison of the measured sequences or peptide mass fingerprints with the NCBI green plant protein database. Type of response, as indicated by Roman numerals, is described in the text. No. Pep., Number of peptides.

Spot No.	Accession No.	Name of Protein	Protein Identification Data
Spot No.	Accession No.	Name of Protein				MALDI (Score/No. Pep.)	LC-MS/MS(Score/No. Pep.)	Theoretical kD/pI	Observed kD/pI	Peptide Sequence (MS/MS)	Response
1	XP_472335	OSJNBa0039C07.4 protein	9.7/18	12.71/1	98.4/5.98	87.2/5.79	(K)TAIAEGLAQR(I)	II
2	S58083	TK	9.79/12	12.07/6	75/6	72.3/7.05	(R)FLAIDAVEK(A)	III
							(K)NGNTGYDEIR(A)
							(K)VTTTIGFGSPNK(A)
							(K)ANSYSVHGSGLGAK(E)
							(K)ALPTYTPESPADATR(N)
							(R)KTPSILALSR(Q)
4	BAA77604	Plastidic ALD	5.69/4	12.77/3	42.5/7.48	35.4/7.23	(K)YTGEGESEEAK(K)	No change
							(R)SAAYYQQGAR(F)	No change
							(K)YTGEGESEEAKK(G)
5	AAO23981	Vacuolar H⁺-ATPase A2 subunit	8.71/11	10.28/2	68.6/5.37	68.7/5.54	(R)NLEDETR(-)	I
		Vacuolar H⁺-ATPase A2 subunit					(R)SGDVYIPR(G)
6	Q40565	RCA	10.13/12	11.62/5	48.3/8.17	40.3/5.02	(R)TDNVPEEAVIK(I)	II
							(R)VYDDEVRK(W)
							(K)WVSGTGIEAIGDK(L)
							(K)LLNSFDGPPTFEQPK(M)
							(R)VQLAETYLK(E)
7	AAG59585	TD	12.43/10	12.72/6	65.6/6.21	64.5/5.94	(R)VYDVAIDSPLQNAAK(L)	I
							(R)EDMQSVFSFK(L)
							(K)DVYDEGR(N)
							(K)QALVLYSVGVNTK(S)
							(K)SSQLNTVNLTNNNLVK(E)
							(K)FLDAFSPR(W)
8	AAM62737	QR-like protein		12.77/1	32.7/6.08	63/5.56	(K)VVAAGLNPVDAKR(R)	III
	AAB39828	Chaperonin		11.51/1	18.10/10.2		(K)IVNDGVTVAR(E)
9	Q40565	Rubisco activase		11.62/11	48.3/8.17	39.1/5.25	(K)GLVQDFSDDQQDIAR(G)	II
							(R)QYNMDNTLDGFYIAPSFMDK(L)
							(K)VPLILGMWGGK(G)
							(R)KMGINPIMMSAGELESGNAGEPAK(L)
							(K)MGINPIMMSAGELESGNAGEPAK(L)
							(R)VYDDEVR(K)
							(R)KWMSGTGIEAIGDK(L)
							(K)LLNSFDGPPTFEQPK(M)
							(K)LLEYGNMLVQEQENVKR(V)
							(K)RVQLAETYLK(E)
							(K)EAALGDANADAINTGNF(-)
12	2002393A	Oxygen-evolving complex protein	5.17/4	11.46/3	26.48/49	29.8/4.83	(R)RLTFDEIQSK(T)	II
		Oxygen-evolving complex protein					(K)FCLEPTSFTVK(A)
							(R)GGSTGYDNAVALPAGGR(G)
13	AAL71857	DHAR	4.07/4		23.6/8.2	22.8/8.3		I
14	BAB64833	PGM	6.02/7	10.4/2	61/6.3	63.7/6.17	(K)LVDLALASGK(I)	III
							(R)LDQVQLLLK(G)
16	AAP04394	Glutathione S-transferase		12.69/1	1/4.07	23.6/8.37	(-)LLDVYETR(-)	I
17	Q42961	Phosphoglycerate kinase precursor	5.2/12		50.1/8.75	41.6/6.99		V
18	NP_199203	Myosin heavy-chain MYA2	1.9/4		169.8/7.9	20.5/8.08		I
19	BAA77604	Plastidic ALD	5.71/5		42.5/7.4	35.9/6.37		V
		Unspecified		12.36/1	1.4/6.93		(-)GAVQLWKYLEDR(-)
20	Q40565	Rubisco activase	8.95/16	14.54/7	48.3/8.4	37.8/7.19	(K)LVVHITK(N)	I
							(K)SFQCELVFR(K)
							(R)EAAEIIR(K)
							(R)EAAEIIRK(G)
							(R)TDNVPEEAVIK(I)
							(R)VYDDEVRK(W)
							(R)VQLAETYLK(E)
21	AAT38067	Putative glycoprotease	1.2/4		40.8/6.19	45.1/<4.55		I
		Unspecified		12.72/1	0.9/11.7		(-)VVKKKGFR(-)
22	AAS90324	3-Dehydroquinate dehydratase	5.31/4		56.1/6.08	32.4/7.93		I
		Unspecified		12.72/1	1.1/9.71		(-)NAGANAYAALTK(-)
23	BAD28273	Hypothetical protein	5.38/4		22.2/9.7	35.3/>9.3		IV
24	AAM97013	Unknown protein Arabidopsis	7.37/5		113.5/9.06	26.6/4.67		I
	BAD32968	RNA-binding protein-like		12.77/1	35.6/10.4		(R)YADPVQR(E)
25	Q9M5M6	60S ribosomal protein L30	3.62/4		12.2/9.6	87.2/8.48		IV
		Putative subtilase		11.6/1	1.2/9.71		(-)TVNTVGAGAATYK(-)
27		Unspecified		12.72/1	1.5/8.89	23.9/4.8	(-)LKPDPNTCGGKDFK(-)	IV
28	S72477	Probable cinnamyl-alcohol dehydrogenase		12.72/1	38.3/5.86	38.9/8.51	(R)DTSGVLSPFNFSR(R)	I
29	AAB33843	Aldehyde dehydrogenase	4.95/4		52.6/5.64	55/7.14		I
30	CAA79702	Mitochondrial formate dehydrogenase precursor	8.54/7	11.59/6	41.8/7.08	37/8.5	(K)IVGVFYK(A)	I
		Mitochondrial formate dehydrogenase precursor					(K)TVGTVGAGR(I)
							(K)MDSELENQIGAK(F)
							(K)FEEDLDK(M)
							(K)GVLIVNNAR(G)
							(K)DGELAPQYR(-)
32	AAM28874	ACS7	4.53/4		77.2/6.51	35.3/8.89		IV
34	P43282	S-adenosylmethionine synthetase	8.4/4	11.38 /2	42.6/6.07	46.3/7.14	(K)SVVASGLAR(R)	I
		S-adenosylmethionine synthetase					(K)TQVTVEYK(N)
35	CAF22093	Glyceraldehyde-3-P dehydrogenase		12.21/2	19.59/8.82	35/8.84	(R)AASFNIIPSSTGAAK(A)	II
		Glyceraldehyde-3-P dehydrogenase					(K)VVISAPSK(D)
37	BAD52936	Putative homocysteine S methyltransferase 4	4.73/5		34.5/6.09	17.9/8.85		IV
		Unspecified		12.72/1	1.2/6.75		(-)QLMDAATTTNAK(-)
38	AAM65239	Unknown protein	5.74/4		32.3/10.1	32.6/7.96		V
	1SM4A	Ferredoxin Nadp reductase		11.22/2	33.3/6		(R)LYSIASSAIGDFGDSK(T)
		Ferredoxin Nadp reductase					(K)DNTFVYMCGLK(G)
39	NP_054979	Hypothetical protein SpolCp075	2.32/4		7/10.8	35.3/>9.3		I
40	AAF27063	F4N2.23	4.38/4		95.3/6.22	37.6/5.68		V
42	AAM46780	Latex plastidic ALD	6.51/4		42.6/8.6	35.1/5.91		V
43	BAA01279	O-acetylserine thiol lyase	3.89/4		34.1/5.61	19.6/8.08		I
45	CAA75381	Translation elongation factor	5.34/10	13.28/1	27.2/5.09	42/4.64	(R)NTTVTGVEMFQK(I)	I
46	Q40565	Rubisco activase	7.82/13	12.72/13	34.36/6.76	35.1/6.14	(K)IVVHITK(N)	I
							(K)SFQCELVFR(K)
							(K)SFQCELVFRK(M)
							(R)YREAAEIIR(K)
							(R)EAAEIIRK(G)
							(K)FYWAPTR(E)
							(R)VYDDEVR(K)
							(R)VYDDEVRK(W)
							(K)WVSGTGIEAIGDK(L)
							(K)LLNSFDGPPTFEQPK(M)
							(K)LLEYGNMLVQEQENVK(R)
							(K)LLEYGNMLVQEQENVKR(V)
							(R)VQLAETYLK(E)
47	P49319	Catalase isozyme 1 salicylic acid-binding protein	5.15/18		56.7/7.02	54.9/8.64		V
48	AAD39604	F23M19.3	2.69/4		67/9.5	73.2/9.09		V
49	CAD27635	Maturase	4.22/4		61.2/9.68	24.5/>9.3		IV
50	BAA24535	Spermidine synthase	5.81/8	−13.9/1	34.4/5.3	35/4.55	(K)ASFCLPSFAK(R)	I
51	CAA78703	Rubisco activase	8.99/8	14.54/6	48.3/8.4	33/6.16	(K)SFQCELVFR(K)	I
							(R)TDNVPEEAVIK(I)
							(R)VYDDEVR(K)
							(K)LLNSFDGPPTFEQPK(M)
							(R)VQLAETYLK(E)
							(K)EAALGDANADAINTGNF(-)
53	CAE05740	OSJNBb0017I01.20	1.68/4		40.8/5.7	24.5/>9.3		IV
54	AAG40343	Hydroxymethyltransferase	5.94/4		51.7/7.6	55/8.73		IV
55	NP_918618	OSJNBa0094H06.18	2.33/4		5.6/10.1	22/>9.3		IV
57	AAF97728	Potassium-sodium symporter	2.52/4		61.9/9.13	38.9/7.53		I
58	NP_915483	P0446B05.25	5.04/4		38.6/9.6	42.1/8.5		I
60	XP_470450	Unknown protein	3.86/4		48.2/10.5	37/7.37		I
61	T07032	S-2-hydroxy acid oxidase	6.05/7		31.2/9.57	44.5/>9.3		I
62	XP_468473	Putative cytochrome P450	5.2/4		58.1/9.4	41.7/5.89		V
64	BAD03386	Hypothetical protein	3.33/4		20.8/10	107.3/<4.5		IV
65	CAA96433	PME	5.1/4	11.46/1	20.1/9.29	37/8.31	(R)VGADMSVINR(C)	IV
66	CAC80376	Glyceraldehyde-3-P dehydrogenase	5.81/4	12.71/5	34/5.56	36.2/8.33	(K)TLLFGEK(S)	II
		Glyceraldehyde-3-P dehydrogenase					(K)KVVISAPSK(D)
							(R)AASFNIIPSSTGAAK(A)
							(K)EASYDDIK(A)
							(K)AAIKEESEGK(L)
67	AAN40027	Hypothetical protein	3.48/5		64.7/9.09	37.9/<4.5		V
68	P29790	ATP synthase γ-subunit	9.47/7		41.4/8.4	35/8.64		IV
	CAB39974	Glyceraldehyde-3-P dehydrogenase		12.72/5	34.4/6.15		(K)DAPMFVVGVNEK(E)
		Glyceraldehyde-3-P dehydrogenase					(R)AASFNIIPSSTGAAK(A)
							(K)VLPSLNGK(L)
							(K)EATYDEIK(A)
							(K)AAIKEESEGK(L)
69	AAF03099	NADP-dependent glyceraldehyde phosphate dehydrogenase		11.71/1	11.8/10.8	31/7.93	(K)DSPLDVIAINDTGGVK(Q)	IV
70	AAG23799	Glyceraldehyde-3-P dehydrogenase		12.66/3	18.2/6.1	35.1/8.73	(R)AAALNIVPTSTGAAK(A)	V
		Glyceraldehyde-3-P dehydrogenase					(K)AVALVLPSLK(G)
							(K)TFAEEVNAAFR(E)
71	AAM62933	Putative chloroplast 50S ribosomal protein L6		14.54/4	24.6/10.2	17.4/>9.3	(R)TLTDNMVVGVSK(G)	V
		Putative chloroplast 50S ribosomal protein L6					(R)ITVSGYDK(S)
							(K)SEIGQFAATVR(K)
							(K)YADEIVR(R)
72	2119042	Gly-rich RNA-binding protein	10.74/9	14.54/3	15.6/5.62	15/5.11	(R)NITVNEAQSR(G)	II
		Gly-rich RNA-binding protein					(R)EGGYGGGGGYGGGR(R)
							(R)EGGYGGGGGGGYGGGR(R)
74	P81665	Oxygen-evolving enhancer protein 1	11.12/9	12.72/2	26.39/4.31	30.2/<4.55	(R)GSSFLDPK(G)	II
		Oxygen-evolving enhancer protein 1					(R)GGSTGYDNAVALPAGGR(G)
75	P81665_1	Oxygen-evolving enhancer protein		12.49/1	1.09/4.07	30.2/<4.55	(K)NAPPEFQK(T)	II
77	T45856	Hypothetical protein F3A4.110	4.26/4		57.2/9.46	30/<4.55		V
	AAP03871	Oxygen-evolving complex 33-kD PSII protein		12.71/6	35.17/5.4		(K)RLTFDEIQSK(T)
		Oxygen-evolving complex 33-kD PSII protein					(R)LTFDEIQSK(T)
							(K)FCLEPTSFTVK(A)
							(K)DGIDYAAVTVQLPGGER(V)
							(R)GGSTGYDNAVALPAGGR(G)
							(R)GDEEELEKENVK(N)
79	B24430	Glyceraldehyde-3-P dehydrogenase	6.51/8	10.85/4	41.78/8.56	41.6/8.45	(K)YDSMLGTFK(A)	V
		Glyceraldehyde-3-P dehydrogenase					(K)IVDNETISVDGK(H)
							(K)GTMTTTHSYTGDQR(L)
							(R)AAALNIVPTSTGAAK(A)
80	AAR96006	4-Nitrophenylphosphatase-like protein		11.68/1	16.5/5.38	29.6/5.18	(R)LVFVTNNSTK(S)	V
82	1909374A	Rubisco activase	7.01/11		42.7/5.57	41/5.59		II
83	AAO62992	Chloroplast Gln synthetase		12.73/2	20.9/5.82	41.7/5.89	(K)AAQIFSDSK(V)	V
							(R)DISDAHYK(A)
84	AAA34111	Rubisco	7.71/4		10.1/5.36	14.8/6.37		V
85	AAG14029	NADH dehydrogenase	3.86/4		37.4/9.69	27/>9.3		II
87	BAA31510	Ribosomal protein L4 chloroplast	2.85/4		31.2/6.59	27.6/8.71		II
89	BAD35631	Putative zinc-finger and C2 domain protein	3.56/4		35.6/6.47	30.9/>9.3		II
90	AAM64724	Unknown protein	3.22/4		61.7/5.23	87.9/<4.5		V
91	BAD15634	Mitochondrial transcription termination factor-like	3.65/4		42.6/9.9	35.9/6.37		V
92	CAB87677	Putative protein	4.78/5		34.7/6.56	42.2/<4.5		II
93	AAR20278	Maturase	6.32/5		55.1/10.4	42.9/4.55		V
94	AAO32311	Putative chloroplast 50S ribosomal protein L6	5.29/6		24.9/10.3	18.9/>9.3		III
95	CAA78703	Rubisco activase	9.14/12	11.62/6	48.3/8.4	41.3/4.71	(K)GLVQDFSDDQQDIAR(G)	II
							(R)EAAEIIR(K)
							(R)TDNVPEEAVIK(I)
							(R)VYDDEVR(K)
							(K)WVSGTGIEAIGDK(L)
							(R)VQLAETYLK(E)
97	NP_174644	Hypothetical protein	2.74/4		21.6/7.7	19.7/<4.5		V
98	BAD28931	Hypothetical protein	2.39/5		7.4/10.3	39.7/8.6		V
99	AAF76363	I box-binding factor	2.77/4		23.9/8.31	42.7/>9.3		V
100	BAD53039	Hypothetical protein	2.88/4		7.3/6.47	41/8.73		V
101	XP_466540	Putative speckle-type POZ	4.05/8		38.8/10.5	76.2/5.25		V
103	P29302	PSI chain II D2 precursor	5.71/5		22.4/10.1	41.5/9		III
105	CAA71589	Protein g5bf	3.82/4	42.6/3.82	37/8.48			III
108	AAU03361	PS2 oxygen-evolving complex protein 3		14.51/1	0.95/10.9	15/>9.3	(-)GKLKGSGKGK(-)	II
109	O23787	Thiazole biosynthetic enzyme precursor	9.53/11	11.62/1	37.5/5.2	32.9/4.8	(K)ALDMNSAEDAIVR(L)	IV