Table II.
Changes of proteins in N. attenuata leaves upon several induction treatments
The accumulations of N. attenuata leaf proteins exhibiting consistent up- or down-regulation (indicated by arrows) at a minimum of three time points (6–72 h) after OS treatment are listed with their P values of water control (W + W), OS (W + OS), FACs (W + FAC) induction, OS without FACs (W + OS-FAC-free), and caterpillar-feeding induction (Larvae) at 48 h. The protein names represent the database identification of the mass spectrometric analyses with the highest confidence. Numbers indicate P values; n/a, P value is not available; –, no change; and x, spot not detectable. qltv, Qualitative change in protein spots.
| Spot No. | Accession No. | Name of Protein | Induction Pattern and P Values for the Treatments
|
||||
|---|---|---|---|---|---|---|---|
| W + W | W + OS | W + FAC | W + OS-FAC-Free | Larvae | |||
| Photosynthesis and photorespiration-related metabolism | |||||||
| 6 | CAA78703 | Rubisco activase | – | ↓ 0.19 | ↓ 0.93 | ↓ 0.11 | ↓ 0.07 |
| 9 | XP_473501, Q40565 | OSJNBb0078D11.3, Rubisco activase | – | ↓ 0.04 | ↓ 0.21 | ↓ 0.003 | ↓ 0.05 |
| 12 | Q40459 | Oxygen-evolving enhancer protein 1 | – | ↓ 0.31 | – | ↓ 0.45 | – |
| 20 | CAA78703 | Rubisco activase | – | ↑ qltv | – | ↑ 0.18 | – |
| 46 | CAA78703 | Rubisco activase | ↑ 0.01 | ↑ qltv | ↑ 0.05 | ↑ 0.15 | ↑ 0.07 |
| 51 | CAA78703 | Rubisco activase | ↑ 0.02 | ↑ qltv | – | ↑ 0.18 | ↑ 0.19 |
| 74 | P81665 | Oxygen-evolving enhancer protein 1 | – | ↓ 0.05 | ↓ 0.11 | – | ↑ 0.06 |
| 75 | P81665_1 | Oxygen-evolving enhancer protein 1 | – | ↓ 0.11 | ↓ 0.15 | ↓ 0.62 | – |
| 77 | AAP03871 | Oxygen-evolving complex 33-kD PSII protein | – | ↓ 0.05 | ↓ 0.12 | – | – |
| 82 | 1909374A | Rubisco activase | – | ↓ 0.27 | ↓ 0.58 | ↓ 0.02 | ↓ 0.12 |
| 95 | CAA78703 | Rubisco activase | ↑ 0.06 | ↓ 0.27 | ↓ 0.41 | ↓ 0.02 | – |
| 108 | AAU03361 | Oxygen-evolving complex protein 3 | – | ↓ 0.01 | – | – | – |
| Primary metabolism | |||||||
| 1 | XP_472335 | OSJNBa0039C07.4 | – | – | – | ↑ 0.09 | ↑ 0.15 |
| 2 | S58083 | TK | ↑ 0.04 | ↑ 0.77 | – | ↑ 0.18 | – |
| 5 | AAO23981 | Vacuolar H+-ATPase A2 subunit | – | ↑ 0.13 | ↑ 0.08 | – | ↑ 0.02 |
| 7 | AAG59585 | TD | ↑ 0.17 | ↑ 0.24 | ↑ 0.34 | – | ↑ 0.02 |
| 21 | AAT38067 | Putative glycoprotease | – | ↑ qltv | – | – | – |
| 29 | AAB33843 | Aldehyde dehydrogenase | – | ↑ 0.12 | ↑ 0.28 | x n/a | – |
| 30 | CAA79702 | Mitochondrial formate dehydrogenase precursor | – | ↑ 0.35 | ↑ 0.18 | – | – |
| 32 | AAM28874 | ACS7 | – | ↑ 0.22 | ↑ 0.27 | ↑ n/a | ↑ n/a |
| 34 | AAO85809 | S-adenosylmethionine synthetase | – | ↑ 0.25 | ↑ 0.06 | ↑ 0.11 | ↑ 0.03 |
| 35 | CAF22093 | Glyceraldehyde-3-P dehydrogenase | – | ↓ 0.8 | – | - n/a | ↑ 0.004 |
| 43 | BAA01279 | O-acetylserine thiol lyase | – | ↑ qltv | ↑ 0.11 | ↑ 0.23 | ↑ 0.18 |
| 50 | BAA24535 | Spermidine synthase | ↑ 0.09 | ↑ 0.08 | ↑ 0.01 | ↑ n/a | – |
| 66 | BAC87864 | Glyceraldehyde-3-P dehydrogenase | – | ↓ 0.26 | – | – | ↓ 0.08 |
| 83 | AAO62992 | Chloroplast Gln synthetase | – | – | – | – | ↑ 0.03 |
| Transcription and translation | |||||||
| 45 | BAA02028 | Translation elongation factor | – | ↑ qltv | – | – | – |
| 72 | BAA03741 | Gly-rich RNA-binding protein | – | ↓ 0.02 | – | ↓ 0.03 | - 0.51 |
| 87 | BAA31510 | Ribosomal protein L4 chloroplast | – | ↓ qltv | – | - n/a | - n/a |
| Secretory pathway | |||||||
| 57 | AAF97728 | Potassium-sodium symporter | ↑ 0.08 | ↑ 0.11 | ↑ 0.04 | ↑ 0.008 | - n/a |
| Secondary metabolism | |||||||
| 8 | AAM62737 | QR-like protein | – | – | ↓ 0.37 | ↓ 0.005 | ↓ 0.21 |
| 22 | AAS90324 | 3-dehydroquinate dehydratase | ↑ 0.21 | ↑ 0.002 | – | – | ↑ 0.77 |
| 24 | BAD32968 | RNA-binding-like protein | ↑ 0.18 | ↑ 0.05 | ↑ 0.02 | ↑ 0.04 | ↑ 0.003 |
| Unspecified function and unknown proteins | |||||||
| 58 | NP_915483 | P0446B05.25 | – | ↑ 0.08 | ↑ 0.25 | – | – |
| 92 | CAB87677 | Putative protein | – | ↓ 0.04 | – | – | – |