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. 2006 Dec;142(4):1493–1510. doi: 10.1104/pp.106.087452

Table IV.

Arabidopsis proteins whose abundance varied significantly between D and ND seeds imbibed for 1 d in basal medium (D 1 d/ND 1 d)

AGI, Accession gene identification; Cov., coverage; Exp., experimental; MM, molecular mass in kD; Theo., theoretical.

No. Exp. MM Exp. pI Arabidopsis Protein Name and Family Cov. % Theo. MM Theo. pI AGI No. Relative Abundanced D 1 d/ND 1 d
Storage protein
    68b 61.86 6.62 Similarity to 7S legumin 24 55.06 6.64 At3g22640 ≥100
    69b 60.20 6.6 Similarity to 7S legumin 30 55.06 6.64 At3g22640 ≥100
    66b 57.58 6.12 12S-3 cruciferin precursor 47 58.24 6.53 At4g28520 4.46 ± 0.22
    131b 52.93 7.31 12S-1 cruciferin precursor 51 52.60 7.68 At5g44120 ≤0.01
    132c 52.99 7.93 12S-1 cruciferin precursor 48 52.60 7.68 At5g44120 ≤0.01
    133c 52.07 8.4 12S-1 cruciferin precursor 48 52.60 7.68 At5g44120 ≤0.01
    134c 53.16 8.57 12S-1 cruciferin precursor 51 52.60 7.68 At5g44120 ≤0.01
    135c 52.35 9.13 12S-1 cruciferin precursor 42 52.60 7.68 At5g44120 ≤0.01
    188b 24.94 8.23 α-Chain of 12S-2 cruciferin 29 27.24 6.34 At1g03880 ≥100
    178b 17.29 5.64 β-Chain of 12S-2 cruciferin 12 20.24 6.36 At1g03880 0.42 ± 0.1
Metabolism and energy
    76c 64.44 6.69 Isocitrate lyase 19 64.24 6.72 At3g21720 0.4 ± 0.1
Chaperone and protein of stress
    20c 71.11 5.3 Putative HSP 70 24 70.91 5.3 At1g16030 ≤0.01
    27c 64.21 5.67 Chaperonin β precursor 29 64.19 6.21 At1g55490 2.30 ± 1.40
    562a 17.94 5.50 HSP17.7 42 17.83 5.22 At5g12030 1.93 ± 0.3
    906a 17 7.78 Peptidyl-prolyl cis-trans isomerase 41 18.60 7.83 At4g38740 ≥100
    476a 17.66 5.25 Dehydrin 6 18.44 7.95 At5g66400 2.11 ± 0.14
    480a 17.42 5.39 Dehydrin 6 18.44 7.95 At5g66400 2.74 ± 0.25
Photosynthesis
    9c 60.07 5.29 Chloroplastic ATP synthase alpha chain 22 55.33 5.19 AtCg00120 3.4 ± 0.6
Amino acid metabolism
    119c 44.83 7.37 Mitochondrial aspartate aminotransferase 30 47.76 8.36 At2g30970 2.71 ± 0.45
Translation protein
    86b 35.48 5.29 60S acidic ribosomal protein PO-B 33 34.14 4.99 At3g09200 3.94 ± 2.02
Unclassified protein
    929a 29.91 5.68 Dormancy related protein 22 59.8 12.5 At1g54870 ≥100
    176c 17.65 5.43 Probable major latex protein 27 17.62 5.91 At4g23670 ≥100
    928a 13.95 6.33 Major latex protein 25 17.86 7.18 At1g14940 ≥100
a

Listed proteins correspond to proteins previously identified in Ler and listed in Rajjou et al. (2006a).

b

Listed proteins correspond to proteins presently identified in Cvi and previously identified in Ler (Rajjou et al., 2006a).

c

Proteins specifically found in Cvi. The peptide sequences of proteins identified in Cvi are available in Supplemental Table S5.

d

Normalized spot volumes in the D imbibed seeds divided by the normalized spot volume in the ND imbibed seeds ± sd: (D 1 d/ND 1 d); ≥100 means that the accumulation level of the corresponding protein in the ND imbibed seeds was close to background; ≤0.01 means that the accumulation level of the corresponding protein in the D imbibed seeds was close to background. Normalized spot volumes from three different gels and independent extractions. Relative abundance observed was statistically significant.