Figure 2.

siz1 increases thermosensitivity during seed germination. Stratified wild-type, siz1-2, siz1-3, NahG siz1-2, NahG, and hot1-3 seeds (3 d in the dark at 4°C) were immediately subjected to heat shock treatment of 45°C for 4 h or incubated at 24°C (normal), sown onto plates, and then maintained under a 16-h daily photoperiod at 24°C. Germination was assessed at the indicated intervals. A, Illustration of representative seeds/seedlings 6 d after heat treatment and B, seed germination data from three independent experiments, mean ± se, n = 21.