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Heat shock-induced SUMO1/2 conjugation is suppressed by siz1-2 and siz1-3. Ten-day-old wild-type, siz1-2, siz1-3, and hot1-3 seedlings were exposed to a 30-min heat shock treatment (39°C, dark, 60% relative humidity). Total protein was extracted from untreated or heat shock-treated seedlings. Twenty micrograms of protein were loaded onto an SDS-PAGE, and the immunoblot was probed with anti-SUMO1, which detects both SUMO1 and SUMO2 (Kurepa et al., 2003).
