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Heat stress-induced SUMO1/2 conjugation/deconjugation is impaired in NahG and hot1-3 seedlings. Ten-day-old wild-type, NahG, and hot1-3 seedlings were exposed to a 39°C heat stress for 15 or 30 min, returned to 24°C, and collected at indicated time points. Black arrow indicates the serial of heat shock. Total protein was extracted as described in Figure 5. Ten micrograms of protein were separated by SDS-PAGE, and the immunoblot was probed with anti-SUMO1.
