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. 2006 Dec;142(4):1728–1738. doi: 10.1104/pp.106.085571

Figure 2.

Figure 2.

A, Graphic representation of the AluI CAPS marker (Ala-132 site) for FRL2 and an example of how AluI cleaves the Ler-specific PCR fragment. B, CAPS marker-based analysis of FRL2 alleles in a pool of 17 and in three individual very early flowering Col/Ler-FRI-Sf2 F2 progeny plants from the cross of Ler-FRI-Sf2 × Col-FRI-Sf2 (#1–#17 and #1, #3, and #5, respectively) and in randomly selected very early flowering F2 plants (1, 2, and 3), late-flowering F2 plants (40, 41, and 44), and intermediate to late-flowering F2 plants (14, 15, and 16) from the cross of Col/Ler-FRI-Sf2#1 × Ler-FRI-Sf2. C, RT-PCR analysis of FRL2 expression levels in Col, Col-FRI-Sf2, Ler, Ler-FRI-Sf2, and Col/Ler-FRI-Sf2. ACTIN2/8 (ACT) is shown as control for loading.