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Introducing a genomic copy of FRL2-Ler into Col/Ler-FRI-Sf2#1 restores FRI-mediated up-regulation of FLC. RT-PCR analysis of FLC expression in FRL2-Ler-transformed T1 lines 19, 22, and 25 and control plants. C, Col alleles of FRL genes; L, Ler alleles of FRL genes. Functional alleles are shown in bold italics. ACTIN2/8 (ACT) was included as loading control.
