Figure 1.

Plant phenotypes associated with T-DNA insertions in AtCRS1 (At5g16180), AtCAF1 (At2g20020), and AtCAF2 (At1g23400). A, Schematic maps of T-DNA insertion sites. Black rectangles are exons and lines are introns. The T-DNA insertion in Atcaf1-2 is within intron 1, 46 nt downstream of that in Atcaf1-1. The sequence flanking each insertion is shown in Supplemental Figure S1. LB, T-DNA left border. B, Seedling phenotypes conditioned by mutant alleles and allele combinations. Where one allele is listed, seedlings are homozygous for the indicated allele. Where two alleles are listed, the plants are the noncomplementing progeny from a cross between plants heterozygous for each allele. Genotypes were confirmed by PCR. Bar=5 mm. C, Segregation of aborted seeds in siliques from a plant heterozygous for Atcaf2-1. The aborted seeds are presumed to be homozygous for the Atcaf2-1 insertion because no homozygous mutants were recovered among the 20 germinating progeny that were genotyped by PCR. The two siliques represent different stages of seed maturation.