Figure 2.

Accumulation of representative subunits of photosynthetic enzyme complexes in AtCRS1, AtCAF1, and AtCAF2 mutants. Duplicate immunoblots of total leaf extract (5 μg protein or the indicated dilution of the wild-type sample) were probed with antibodies to the indicated proteins. The mutant plants were homozygous for the indicated allele. The bottom section shows the filter that had been probed for PsbA stained with Ponceau S, to demonstrate similar protein loading, and to detect the large subunit of Rubisco (RbcL). OE23 and OE33 are luminal proteins found in the oxygen-evolving complex associated with PSII. Bands corresponding to the putative precursor (p) and stromal intermediate (i) forms of OE23 are marked.