Figure 4.

Analysis of vascular tissue-specific expression using macroarrays. PCR-derived fragments of 216 different ESTs were spotted in duplicate on nylon filters and hybridized to radiolabeled mRNA isolated from vascular or nonvascular tissue. The relative expression intensities in vascular and nonvascular tissue are presented (±sd; n = 3). A, Genes with high expression levels. Bars marked with one asterisk represent mRNAs of MTs; the bar marked with two asterisks shows a vascular-specific aquaporin (PmAQP1); the bar marked with three asterisks represents a GRP (PmPSP1). B, Genes with medium expression levels. The bar marked with one asterisk represents PmAAMY1, an α-amylase gene; the bar marked with two asterisks shows PmSPS1, the gene of a Suc phosphate synthase. C, Genes with low expression levels. A to C, Thin arrows mark mRNAs from the functionally uncharacterized proteins group; thick and white arrows mark mRNAs from the novel genes group.