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Farnesylation does not affect subcellular localization of AtNAP1;1. A, GFP-AtNAP1;1, GFP-AtNAP1;1C369S, GFP-CaM53, GFP-CaM53mS, and GFP proteins were detected by green fluorescence using confocal laser scanning microscopy in epidermal onion cells 38 h after bombardment. Bars correspond to 50 μm. B, Localization of GFP-AtNAP1;1 and GFP- AtNAP1;1C369S fusion proteins in tobacco BY-2 tobacco cells. Transgenic suspension of BY-2 tobacco cells were established and used for localization.
