Figure 4.

Arabidopsis Atnap1;1 mutant alleles are affected in cell proliferation during leaf development. A, Atnap1;1 gene structure and location of T-DNA insertions in the Atnap1;1-1 and Atnap1;1-2 mutant alleles. B, Western-blot analysis of AtNAP1 proteins in wild type (lane 1), Atnap1;1-1 (lane 2), Atnap1;1-2 (lane 3), Atnap1;2 (lane 4), and Atnap1;3 (lane 5) mutant alleles. Samples were collected from 9-d-old plants, 25 μg of total protein was loaded per well, and membrane was probed with polyclonal AtNAP1 antibody. C, Nine-day-old sterile-grown plants showing the differences in leaf and cotyledon size. D, Atnap1;1-1 and Atnap1;1-2 average first leaf blade area and average number of cells (E). Twenty individual leaves and 40 palisade mesophyll cells were analyzed in duplicate.