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. 2006 Dec;142(4):1642–1655. doi: 10.1104/pp.106.088476

Figure 5.

Figure 5.

GRIK1 and GRIK2 are degraded by the proteasome. A, Two-week-old Arabidopsis seedlings were treated with MG132 (lane 3) or buffer (lane 2) for 3 h and total proteins were subjected to immunoblot analysis using GRIK1 or GRIK2 peptide antibodies. Seedlings collected immediately before treatment (time = 0) were tested in lane 1. A nonspecific band was used as an internal control. B, Mock-inoculated (M; lanes 1 and 2) or CaLCuV-infected (I; lanes 3 and 4) leaves were excised and treated with MG132 (lanes 2 and 4) or buffer only (lanes 1 and 3) for 3 h, and total proteins were analyzed by immunoblotting with antibodies against GRIK1, GRIK2, or CaLCuV AL1. A nonspecific band served as an internal control. C, CaLCuV-infected leaves were excised and treated for 3 h with cycloheximide (CHX; lane 2), MG132 (lane 3), or buffer (lane 1), and total proteins were analyzed by immunoblotting with antibodies against GRIK1, GRIK2, and CaLCuV AL1.